Analysis Note

ControlPOSITIVE CONTROL:

HeLa or NIH3T3 cells. Germinal center in a tonsil or reactive lymph node. Cellular localization is predominantly nuclear, with some cytoplasmic staining.

Application

Anti-Cdk1 Antibody, clone A17.1.1 is a Mouse Monoclonal Antibody for detection of Cdk1 also known as p34cdc2, CDC2 & has been validated in IF, IP, EA, WB, IHC(P).

Research CategoryEpigenetics & Nuclear Function

Frozen Sections

Immunofluorescence

Immunoprecipitation: (Native/denatured, Protein A; 2µg/mg protein lysate)

Immunohistology (Formalin, 2-4µg/mL for 30 min at RT)

Paraffin Sections (staining requires antigen retrieval).

Western Blotting

Kinase Assay

Optimal working dilutions must be determined by the end user.

p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues. MAB8878 inhibits the activation of p34cdc2 kinase by cyclins and precipitates an active kinase.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Raised against Xenopus p34cdc2.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. 0.2 mg/mL in 10 mM PBS, pH 7.4, containing 0.2% BSA and 0.09% Sodium Azide.

Format: Purified

Specificity

Recognizes a 34 kDa protein cdk1, identified as cdk1/p34cdc2 protein kinase (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. It shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks).

Storage and Stability

Maintain at 2° to 8°C in undiluted aliquots for up to 12 months from date of receipt.